验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, MCF-7 cells, HEK-293 cells, HeLa cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human appendicitis tissue, human colon cancer tissue, mouse stomach tissue, mouse colon tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue, mouse brain tissue |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11085-1-AP targets PARD3 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken |
| 免疫原 |
CatNo: Ag1565 Product name: Recombinant human PARD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 217-497 aa of BC011711 Sequence: RERDYAEIQDFHRTFGCDDELMYGGVSSYEGSMALNARPQSPREGHMMDALYAQVKKPRNSKPSPVDSNRSTPSNHDRIQRLRQEFQQAKQDEDVEDRRRTYSFEQPWPNARPATQSGRHSVSVEVQMQRQRQEERESSQQAQRQYSSLPRQSRKNASSVSQDSWEQNYSPGEGFQSAKENPRYSSYQGSRNGYLGGHGFNARVMLETQELLRQEQRRKEQQMKKQPPSEGPSNYDSYKKVQDPSYAPPKGPFRQDVPPSPSQVARLNRLQTPEKGRPFYS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | par-3 partitioning defective 3 homolog (C. elegans) |
| 别名 | PARD-3, ASIP, Atypical PKC isotype-specific-interacting protein, Baz, Bazooka |
| 计算分子量 | 151 kDa |
| 观测分子量 | 180 kDa, 140-150 kDa, 100 kDa |
| GenBank蛋白编号 | BC011711 |
| 基因名称 | PARD3 |
| Gene ID (NCBI) | 56288 |
| RRID | AB_2159649 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TEW0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts. PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PARD3 antibody 11085-1-AP | Download protocol |
| IHC protocol for PARD3 antibody 11085-1-AP | Download protocol |
| IP protocol for PARD3 antibody 11085-1-AP | Download protocol |
| WB protocol for PARD3 antibody 11085-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater m6 A Reader YTHDF1-Targeting Engineered Small Extracellular Vesicles for Gastric Cancer Therapy via Epigenetic and Immune Regulation | ||
J Exp Clin Cancer Res PARD3 drives tumorigenesis through activating Sonic Hedgehog signalling in tumour-initiating cells in liver cancer | ||
Cell Rep Med Decoding the pathogenesis of spermatogenic failure in cryptorchidism through single-cell transcriptomic profiling | ||
Cell Rep Med Patient-derived kidney organoids recapitulate ADPKD and facilitate the identification of Rho pathway inhibitors as candidate therapeutics. | ||
Cell Commun Signal Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cells. |
