验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55310-1-AP targets PAMR1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | regeneration associated muscle protease |
| 别名 | DKFZP586H2123, FP938, Inactive serine protease PAMR1, PAMR1, RAMP, UNQ699/PRO1344 |
| 计算分子量 | 82 kDa |
| 观测分子量 | 78-82 kDa, 67-70 kDa |
| GenBank蛋白编号 | NM_015430 |
| 基因名称 | PAMR1 |
| Gene ID (NCBI) | 25891 |
| RRID | AB_11232034 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6UXH9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAMR1,also named as RAMP, belongs to the peptidase S1 family. It may play a role in regeneration of skeletal muscle. This antibody is specific to PAMR1.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PAMR1 antibody 55310-1-AP | Download protocol |
| WB protocol for PAMR1 antibody 55310-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol High Expression of PAMR1 Predicts Favorable Prognosis and Inhibits Proliferation, Invasion, and Migration in Cervical Cancer. | ||
J Ethnopharmacol Qizhu Anticancer Prescription enhances immunosurveillance of liver cancer cells by regulating p21-dependent secretory phenotypes | ||
Funct Integr Genomics NEDD4 facilitates the progression of endometrial carcinoma by enhancing PAMR1 protein degradation through ubiquitination |
