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验证数据展示

  • WB analysis of HepG2 using 15321-1-AP (same clone as 15321-1-PBS)

    WB analysis of HepG2 using 15321-1-AP (same clone as 15321-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15321-1-AP (PAM16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15321-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 15321-1-AP (same clone as 15321-1-PBS)

    IHC staining of human ovary cancer using 15321-1-AP (same clone as 15321-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15321-1-AP (PAM16 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15321-1-PBS in a different storage buffer formulation.

PAM16 Polyclonal antibody, PBS Only

PAM16 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15321-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

MAGMAS, CGI-136, TIM16, Presequence translocated-associated motor subunit PAM16, Mitochondrial import inner membrane translocase subunit TIM16

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

15321-1-PBS targets PAM16 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0970

Product name: Recombinant human PAM16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-105 aa of BC005024

Sequence: MAKYLAQIIVMGVQVVGRAFARALRQEFAASRAAADARGRAGHRSAAASNLSGLSLQEAQQILNVSKLSPEEVQKNYEHLFKVNDKSVGGSFYLQSKVVRAKERP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondria-associated protein involved in granulocyte-macrophage colony-stimulating factor signal transduction
别名 MAGMAS, CGI-136, TIM16, Presequence translocated-associated motor subunit PAM16, Mitochondrial import inner membrane translocase subunit TIM16
计算分子量 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC005024
基因名称 PAM16
Gene ID (NCBI) 51025
RRIDAB_2878126
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3D7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAM16, also named as Magmas, TIM16 and TIMM16, is regulates ATP-dependent protein translocation into the mitochondrial matrix. The MW of PAM16 is about 14-16 kDa in WB detection. PAM16 is a component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19.

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