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验证数据展示

  • WB analysis using 87921-1-RR (same clone as 87921-1-PBS)

    WB analysis using 87921-1-RR (same clone as 87921-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87921-1-RR (PAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87921-1-PBS in a different storage buffer formulation.

  • IP experiment of U2OS using 87921-1-RR (same clone as 87921-1-PBS)

    IP experiment of U2OS using 87921-1-RR (same clone as 87921-1-PBS)

    IP result of anti-PAM (IP:87921-1-RR, 4ug; Detection:87921-1-RR 1:1000) with U2OS cells lysate 800 ug. This data was developed using the same antibody clone with 87921-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 87921-1-RR (same clone as 87921-1-PBS)

    IF Staining of U2OS using 87921-1-RR (same clone as 87921-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PAM antibody (87921-1-RR, Clone: 260074A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87921-1-PBS in a different storage buffer formulation.

  • Affinity of 87921-1-RR

    Affinity of 87921-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87921-1-RR against Human PAM were performed. The affinity constant is below 1 pM.

PAM Recombinant monoclonal antibody, PBS Only

PAM Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 87921-1-PBS

产品说明书

CloneNo. 260074A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

PAL, PAM-1, Peptidyl-alpha-hydroxyglycine alpha-amidating lyase, Peptidyl-glycine alpha-amidating monooxygenase, Peptidylglycine alpha-hydroxylating monooxygenase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87921-1-PBS targets PAM in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg5194

Product name: Recombinant human PAM protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 21-494 aa of NM_001177306.2

Sequence: FRSPLSVFKRFKETTRPFSNECLGTTRPVVPIDSSDFALDIRMPGVTPKQSDTYFCMSMRIPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNNKDCSGVSLHLTRLPQPLIAGMYLMMSVDTVIPAGEKVVNSDISCHYKNYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVGHPVDVSFGDLLAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPPEANIPIPVKSDMVMMHEHHKETEYKDKIPLLQQPKREEEEVLDQGDFYSLLSKLLGEREDVVHVHKYNPTEKAESESDLVAEIANVVQKKDLGRSDAREGAEHERGNAILVRDRIHKFHRLVSTLRPPESRVFSLQQPPPGEGTWEPEHTG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 peptidylglycine alpha-amidating monooxygenase
别名 PAL, PAM-1, Peptidyl-alpha-hydroxyglycine alpha-amidating lyase, Peptidyl-glycine alpha-amidating monooxygenase, Peptidylglycine alpha-hydroxylating monooxygenase
计算分子量108 kDa
观测分子量75 kDa and 105-120 kDa
GenBank蛋白编号NM_001177306.2
基因名称 PAM
Gene ID (NCBI) 5066
RRIDAB_3745828
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP19021-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Peptidyl-glycine alpha-amidating monooxygenase (PAM) is a bifunctional type I membrane protein essential for neuropeptide maturation. It catalyzes the C-terminal amidation of peptides via two sequential enzymatic domains: peptidylglycine α-hydroxylating monooxygenase (PHM, ~44-46 kDa) and peptidyl-α-hydroxyglycine α-amidating lyase (PAL, ~50 kDa), which together convert glycine-extended precursors into bioactive amidated peptides. PAM undergoes alternative splicing, generating integral membrane isoforms such as PAM-1 (~120 kDa) and PAM-2 (~105 kDa). Endoproteolytic cleavage produces soluble monofunctional PHM and PAL, as well as a bifunctional ~75 kDa fragment. The cleaved cytosolic domain (sfCD, ~16 kDa) can translocate to the nucleus, influencing gene expression. PAM also supports angiogenesis and tumor progression, notably in glioblastoma, highlighting its therapeutic potential (PMID: 8436961; PMID: 32054826).

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