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验证数据展示

  • WB analysis using 32085-1-AP (same clone as 32085-1-PBS)

    WB analysis using 32085-1-AP (same clone as 32085-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32085-1-AP (PAK7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32085-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 32085-1-AP (same clone as 32085-1-PBS)

    IHC staining of mouse brain using 32085-1-AP (same clone as 32085-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32085-1-AP (PAK7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32085-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 32085-1-AP (same clone as 32085-1-PBS)

    IHC staining of mouse brain using 32085-1-AP (same clone as 32085-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32085-1-AP (PAK7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32085-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 32085-1-AP (same clone as 32085-1-PBS)

    IF Staining of U-251 using 32085-1-AP (same clone as 32085-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PAK7 antibody (32085-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 32085-1-PBS in a different storage buffer formulation.

PAK7 Polyclonal antibody, PBS Only

PAK7 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 32085-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PAK4/6/7, EC:2.7.11.1, KIAA1264, p21-activated kinase 5, p21-activated kinase 7

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32085-1-PBS targets PAK7 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag29190

Product name: Recombinant human PAK7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 180-360 aa of BC024179

Sequence: AYYSEVKPLKSDFARFSADYHSHLDSLSKPSEYSDLKWEYQRASSSSPLDYSFQFTPSRTAGTSGCSKESLAYSESEWGPSLDDYDRRPKSSYLNQTSPQPTMRQRSRSGSGLQEPMMPFGASAFKTHPQGHSYNSYTYPRLSEPTMCIPKVDYDRAQMVLSPPLSGSDTYPRGPAKLPQS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 7
别名 PAK4/6/7, EC:2.7.11.1, KIAA1264, p21-activated kinase 5, p21-activated kinase 7
计算分子量 719 aa, 81 kDa
观测分子量80 kDa
GenBank蛋白编号BC024179
基因名称 PAK7
Gene ID (NCBI) 57144
RRIDAB_3670187
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P286
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAK7, also known as PAK5, is a newly found but scarcely researched type II PAKs (PAK4, 5, 6). PAK7 was originally found in the brain, which promotes filopodia formation in nerve cells. Recently, studies have found that the expression level of PAK7 significantly increased in colorectal cancer, ovarian cancer, gastric cancer, neuroglioma, hepatocellular carcinoma, pancreatic cancer, osteosarcoma, and breast cancer. PAK7 plays an important role in tumorigenesis, invasion, and metastasis, mainly related to its mechanism of regulating cytoskeleton, inhibiting apoptosis, and promoting proliferation. The calculated molecular weight of PAK7 is 80 kDa (PMID: 29805709, PMID: 34165002).

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