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- KD/KO Validated
PAK4 Polyclonal antibody
PAK4 Polyclonal Antibody for FC, IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, HeLa cells, PC-3 cells, rat skin tissue |
Positive IP detected in | mouse liver tissue |
Positive IF detected in | Hela cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14685-1-AP targets PAK4 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | PAK4 fusion protein Ag5981 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | p21 protein (Cdc42/Rac)-activated kinase 4 |
Synonyms | KIAA1142, p21 activated kinase 4, PAK 4, PAK4 |
Calculated Molecular Weight | 64 kDa |
Observed Molecular Weight | 64 kDa |
GenBank Accession Number | BC025282 |
Gene Symbol | PAK4 |
Gene ID (NCBI) | 10298 |
RRID | AB_2158610 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O96013 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PAK4 antibody 14685-1-AP | Download protocol |
IF protocol for PAK4 antibody 14685-1-AP | Download protocol |
IP protocol for PAK4 antibody 14685-1-AP | Download protocol |
FC protocol for PAK4 antibody 14685-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions.
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Cancer Res Targeting PAK4 Inhibits Ras-Mediated Signaling and Multiple Oncogenic Pathways in High-Risk Rhabdomyosarcoma. | ||
Mol Ther Nucleic Acids ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway. | ||
Aging (Albany NY) PAK4 suppresses TNF-induced release of endothelial microparticles in HUVECs cells.
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Mol Neurobiol Exosomes Derived from Meningitic Escherichia coli-Infected Brain Microvascular Endothelial Cells Facilitate Astrocyte Activation |