验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, pig brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32848-1-AP targets PAK3 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 | PAK3 fusion protein Ag38566 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | p21 protein (Cdc42/Rac)-activated kinase 3 |
| 别名 | Beta-PAK, EC:2.7.11.1, Oligophrenin-3, OPHN3, p21-activated kinase 3 |
| 计算分子量 | 62kDa，559aa |
| 观测分子量 | 64 kDa |
| GenBank蛋白编号 | NM_001128173.2 |
| 基因名称 | PAK3 |
| Gene ID (NCBI) | 5063 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O75914 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAK3, also known as p21-activated kinase 3, is a member of the PAK family of serine/threonine protein kinases, classified as Group I PAK kinases along with PAK1 and PAK2. PAK3 is predominantly expressed in neural cells, especially in regions such as the brain. PAK3 plays a crucial role in dendritic spine development and synaptic plasticity. PAK3 plays an important role in cell migration and morphology. It regulates dynamic changes in the actomyosin cytoskeleton, microtubules, and adhesion interactions with the microenvironment. In neurons and other cell types, PAK3 contributes to cell migration, neurite growth, and dendritic spine morphogenesis by participating in signaling cascades that control cell adhesion and cell motility. Mutations in PAK3 are associated with X-linked non-syndromic intellectual disability.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAK3 antibody 32848-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |