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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

    WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

  • WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

    WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68042-1-Ig (same clone as 68042-1-PBS)

    IHC staining of human liver cancer using 68042-1-Ig (same clone as 68042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68042-1-Ig (PAIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

PAIP1 Monoclonal antibody, PBS Only

PAIP1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68042-1-PBS

产品说明书

CloneNo. 2H9D4

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rabbit

应用

WB, IHC, Indirect ELISA

别名

PABP interacting protein 1, PAIP 1, PAIP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68042-1-PBS targets PAIP1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rabbit samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rabbit
免疫原

CatNo: Ag16773

Product name: Recombinant human PAIP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 102-400 aa of BC005295

Sequence: EQFAETLNGCVTTDDALQELVELIYQQATSIPNFSYMGARLCNYLSHHLTISPQSGNFRQLLLQRCRTEYEVKDQAAKGDEVTRKRFHAFVLFLGELYLNLEIKGTNGQVTRADILQVGLRELLNALFSNPMDDNLICAVKLLKLTGSVLEDAWKEKGKMDMEEIIQRIENVVLDANCSRDVKQMLLKLVELRSSNWGRVHATSTYREATPENDPNYFMNEPTFYTSDGVPFTAADPDYQEKYQELLEREDFFPDYEENGTDLSGAGDPYLDDIDDEMDPEIEEAYEKFCLESERKRKQ

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 poly(A) binding protein interacting protein 1
别名 PABP interacting protein 1, PAIP 1, PAIP1
计算分子量 70 kDa
观测分子量40-45kDa, 62kDa
GenBank蛋白编号BC005295
基因名称 PAIP1
Gene ID (NCBI) 10605
RRIDAB_2918784
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H074
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.