验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, BxPC-3 cells, COS-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10675-1-AP targets PAIP1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, monkey samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, RIP |
| 经测试反应性 | human, monkey |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag1070 Product name: Recombinant human PAIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 102-400 aa of BC005295 Sequence: EQFAETLNGCVTTDDALQELVELIYQQATSIPNFSYMGARLCNYLSHHLTISPQSGNFRQLLLQRCRTEYEVKDQAAKGDEVTRKRFHAFVLFLGELYLNLEIKGTNGQVTRADILQVGLRELLNALFSNPMDDNLICAVKLLKLTGSVLEDAWKEKGKMDMEEIIQRIENVVLDANCSRDVKQMLLKLVELRSSNWGRVHATSTYREATPENDPNYFMNEPTFYTSDGVPFTAADPDYQEKYQELLEREDFFPDYEENGTDLSGAGDPYLDDIDDEMDPEIEEAYEKFCLESERKRKQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | poly(A) binding protein interacting protein 1 |
| 别名 | Polyadenylate-binding protein-interacting protein 1, Poly(A)-binding protein-interacting protein 1, PAIP-1, PAIP 1, PABP-interacting protein 1 |
| 计算分子量 | 70 kDa |
| 观测分子量 | 60 kDa, 51 kDa, 40 kDa |
| GenBank蛋白编号 | BC005295 |
| 基因名称 | PAIP1 |
| Gene ID (NCBI) | 10605 |
| RRID | AB_2160391 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H074 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
In initiation of translation in eukaryotes, binding of the small ribosomal subunit to mRNA requires recognition of the 5-prime cap structure by the cap-binding complex eIF4F. eIF4F consists of eIF4E, eIF4A, and eIF4G. Translation initiation is further regulated by the mRNA 3-prime poly(A) tail and the poly(A)-binding protein (PABC1). PAIP1 interacts with PABC1 and some eIF4 complexes [PMID:9548260]. It is a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs, and associates with EIF4A and PABPC1 may potentiate contacts between mRNA termini [PMID:11051545].
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PAIP1 antibody 10675-1-AP | Download protocol |
| IP protocol for PAIP1 antibody 10675-1-AP | Download protocol |
| WB protocol for PAIP1 antibody 10675-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem J Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity. | ||
Biol Reprod Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2. | ||
Hum Pathol Paip1 predicts poor prognosis and promotes tumor progression through AKT/GSK-3β pathway in lung adenocarcinoma.
| ||
Exp Cell Res Role of Paip1 on angiogenesis and invasion in pancreatic cancer.
| ||
Cancer Res Treat Paip1 Indicated Poor Prognosis in Cervical Cancer and Promoted Cervical Carcinogenesis.
| ||
Arch Biochem Biophys YAP1-activated ZNF131 promotes hepatocellular carcinoma cell proliferation through transcriptional regulation of PAIP1 |
