验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
10085-1-AP targets PAFAH2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag0124 Product name: Recombinant human PAFAH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 165-392 aa of BC001158 Sequence: WIPFRRVEEGEKEFHVRNPQVHQRVSECLRVLKILQEVTAGQTVFNILPGGLDLMTLKGNIDMSRVAVMGHSFGGATAILALAKETQFRCAVALDAWMFPLERDFYPKARGPVFFINTEKFQTMESVNLMKKICAQHEQSRIITVLGSVHRSQTDFAFVTGNLIGKFFSTETRGSLDPYEGQEVMVRAMLAFLQKHLDLKEDYNQWNNLIEGIGPSLTPGAPHHLSSL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | platelet-activating factor acetylhydrolase 2, 40kDa |
| 别名 | EC:2.3.1.149, EC:3.1.1.47, HSD PLA2, hSD-PLA2, PAF:lysophospholipid transacetylase |
| 计算分子量 | 40 kDa |
| 观测分子量 | 44-46 kDa |
| GenBank蛋白编号 | BC001158 |
| 基因名称 | PAFAH2 |
| Gene ID (NCBI) | 5051 |
| RRID | AB_2160055 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99487 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The family of platelet-activating factor-acetylhydrolase (PAF-AH) consists of enzymes which catalyze the hydrolysis of acetyl ester at the sn-2 position of PAF(Platelet-activating factor). The intracellular type II PAF-AH is a single polypeptide about a 40 kd distributed in both the cytosol and membranes of many kinds of cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAFAH2 antibody 10085-1-AP | Download protocol |
| IHC protocol for PAFAH2 antibody 10085-1-AP | Download protocol |
| IP protocol for PAFAH2 antibody 10085-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Oxidatively truncated phospholipids are required agents of tumor necrosis factor α (TNFα)-induced apoptosis. | ||
J Lipid Res Intracellular PAF catabolism by PAF acetylhydrolase counteracts continual PAF synthesis. | ||
Biochemistry Trafficking of platelet-activating factor acetylhydrolase type II in response to oxidative stress. |