验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, NCI-H1299 cells, A549 cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20564-1-AP targets PAFAH1B3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | platelet-activating factor acetylhydrolase, isoform Ib, gamma subunit 29kDa |
| 别名 | EC:3.1.1.47, PAF-AH 29 kDa subunit, PAF-AH subunit gamma, PAFAHG, Platelet-activating factor acetylhydrolase IB subunit alpha1 |
| 计算分子量 | 26 kDa |
| 观测分子量 | 26-29 kDa |
| GenBank蛋白编号 | NM_002573 |
| 基因名称 | PAFAH1B3 |
| Gene ID (NCBI) | 5050 |
| RRID | AB_10693534 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15102 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAFAH1B3, also named as PAFAHG, belongs to the 'GDSL' lipolytic enzyme family and Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily. It inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. PAFAH1B3 plays an important role during the development of brain. The antibody is specific to PAFAH1B3.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PAFAH1B3 antibody 20564-1-AP | Download protocol |
| IHC protocol for PAFAH1B3 antibody 20564-1-AP | Download protocol |
| WB protocol for PAFAH1B3 antibody 20564-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol PAFAH1B3 promotes gastric cancer progression by enhancing E2F8-mediated transcriptional activation of VEGFA expression. | ||
Int J Mol Sci PAFAH1B3 Exists in Linear Chromosomal and Extrachromosomal Circular DNA and Promotes HCC Progression via EMT.
| ||
Discov Oncol Increased PAFAH1B3 was associated with poor prognosis and T-cell exhaustion microenvironment in hepatocellular carcinoma | ||
Discov Oncol Prognostic clinical phenotypes associated with tumor stemness in the immune microenvironment of T-cell exhaustion for hepatocellular carcinoma | ||
Onco Targets Ther Aberrant expression of PAFAH1B3 associates with poor prognosis and affects proliferation and aggressiveness in hypopharyngeal squamous cell carcinoma.
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