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验证数据展示

  • WB analysis using 29169-1-AP

    WB analysis using 29169-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29169-1-AP (PADI2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 29169-1-AP

    IHC staining of human kidney using 29169-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29169-1-AP (PADI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PADI2 Polyclonal antibody

PADI2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29169-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

KIAA0994, PAD H19, PAD2, PADI2, PDI2, Peptidylarginine deiminase II

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29169-1-AP targets PADI2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PADI2 fusion protein Ag29042 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidyl arginine deiminase, type II
别名 KIAA0994, PAD H19, PAD2, PADI2, PDI2, Peptidylarginine deiminase II
计算分子量 665 aa, 75 kDa
观测分子量75 kDa
GenBank蛋白编号BC009701
基因名称 PADI2
Gene ID (NCBI) 11240
RRIDAB_2918243
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2J8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PADI2, also named as KIAA0994, PDI2, PAD-H19 and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.

实验方案

Product Specific Protocols
WB protocol for PADI2 antibody 29169-1-APDownload protocol
IHC protocol for PADI2 antibody 29169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols