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验证数据展示

  • WB analysis using 30172-1-AP (same clone as 30172-1-PBS)

    WB analysis using 30172-1-AP (same clone as 30172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30172-1-AP (PACS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

  • WB analysis of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

    WB analysis of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

    WB result of PACS2 antibody (30172-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PACS2 transfected HCT 116 cells. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

  • IP experiment of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

    IP experiment of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

    IP result of anti-PACS2 (IP:30172-1-AP, 4ug; Detection:30172-1-AP 1:1500) with HCT 116 cells lysate 2440 ug. This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

  • IF Staining of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

    IF Staining of HCT 116 using 30172-1-AP (same clone as 30172-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using PACS2 antibody (30172-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30172-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PACS2 Polyclonal antibody, PBS Only

PACS2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 30172-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30172-1-PBS targets PACS2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32902

Product name: Recombinant human PACS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 340-500 aa of NM_001100913

Sequence: SARSHKEPPSPADVPEKTRSLGGRQPSDSVSDTVALGVPGPREHPGQPEDSPEAEASTLDVFTERLPPSGRITKTESLVIPSTRSEGKQAGRRGRSTSLKERQAARPQNERANSLDNERCPDARSQLQIPRKTVYDQLNHILISDDQLPENIILVNTSDWQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphofurin acidic cluster sorting protein 2
别名
计算分子量98kd
观测分子量100-130 kDa
GenBank蛋白编号NM_001100913
基因名称 PACS2
Gene ID (NCBI) 23241
RRIDAB_3086254
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86VP3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PACS2, also named PACS1L or KIAA0602, is one of PACS (phosphofurin acidic cluster sorting) proteins which are one such family of multifunctional membrane traffic regulators that mediate organ homoeostasis and have important roles in diverse pathologies and disease states. PACS2 is a multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, ER homeostasis, and apoptosis. PACS2 may be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.

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