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PABPC4 Polyclonal antibody

PABPC4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14960-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

Inducible poly(A)-binding protein, APP-1, APP1, APP 1, Activated-platelet protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存


经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, human brain tissue, Jurkat cells
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PABPC4 Ready-To-Use IHC Kit. PABPC4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14960-1-AP targets PABPC4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 PABPC4 fusion protein Ag6813 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly(A) binding protein, cytoplasmic 4 (inducible form)
别名 Inducible poly(A)-binding protein, APP-1, APP1, APP 1, Activated-platelet protein 1
计算分子量 70 kDa
观测分子量 70-71 kDa
GenBank蛋白编号BC065540
基因名称 PABPC4
Gene ID (NCBI) 8761
RRIDAB_2156897
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13310
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PABPC4 antibody 14960-1-APDownload protocol
IHC protocol for PABPC4 antibody 14960-1-APDownload protocol
IF protocol for PABPC4 antibody 14960-1-APDownload protocol
IP protocol for PABPC4 antibody 14960-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Nucleic Acids Res

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.

Authors - Evan P Booy
humanWB,IHC,IP

EMBO Rep

LINC00493-encoded microprotein SMIM26 exerts anti-metastatic activity in renal cell carcinoma

Authors - Kun Meng
  • KD Validated
humanWB

J Immunol

HNRNPR Regulates the Expression of Classical and Nonclassical MHC Class I Proteins.

Authors - Adi Reches
  • KD Validated
mouse

Nat Struct Mol Biol

Poised PABP-RNA hubs implement signal-dependent mRNA decay in development

Authors - Miha Modic
humanWB

BMC Pregnancy Childbirth

Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics.

Authors - Xiaotong Sun
humanWB

PLoS Pathog

Elucidation of TRIM25 ubiquitination targets involved in diverse cellular and antiviral processes

Authors - Emily Yang
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