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PABPC3 Polyclonal antibody

PABPC3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12625-2-AP

Print datasheet

Synonyms

PABP 3, PABP3, PABPC3, PABPL3, Poly(A) binding protein 3

验证数据展示

  • WB analysis using 12625-2-AP

    WB analysis using 12625-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12625-2-AP (PABPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 12625-2-AP

    IHC staining of human testis using 12625-2-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12625-2-AP (PABPC3 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PABPC3 antibody included
  • IHC staining of human testis using 12625-2-AP

    IHC staining of human testis using 12625-2-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12625-2-AP (PABPC3 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PABPC3 antibody included
  • IP experiment of mouse testis using 12625-2-AP

    IP experiment of mouse testis using 12625-2-AP

    IP result of anti-PABPC3 (IP:12625-2-AP, 4ug; Detection:12625-2-AP 1:300) with mouse testis tissue lysate 3600ug.

经过测试的应用

Positive WB detected inrat testis tissue, mouse testis tissue, Jurkat cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PABPC3 Ready-To-Use IHC Kit. PABPC3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12625-2-AP targets PABPC3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PABPC3 fusion protein Ag3317 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name poly(A) binding protein, cytoplasmic 3
Synonyms PABP 3, PABP3, PABPC3, PABPL3, Poly(A) binding protein 3
Calculated Molecular Weight 631 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC027617
Gene Symbol PABPC3
Gene ID (NCBI) 5042
RRIDAB_2156892
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H361
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PABPC3 antibody 12625-2-APDownload protocol
IHC protocol for PABPC3 antibody 12625-2-APDownload protocol
IP protocol for PABPC3 antibody 12625-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

J Assist Reprod Genet

The poly(A)-binding protein genes, EPAB, PABPC1, and PABPC3 are differentially expressed in infertile men with non-obstructive azoospermia.

Authors - Saffet Ozturk
View Article