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验证数据展示

  • WB analysis of Jurkat using 17650-1-AP (same clone as 17650-1-PBS)

    WB analysis of Jurkat using 17650-1-AP (same clone as 17650-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17650-1-AP (PAAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 17650-1-AP (same clone as 17650-1-PBS)

    IF Staining of U2OS using 17650-1-AP (same clone as 17650-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PAAF1 antibody (17650-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 17650-1-AP (same clone as 17650-1-PBS)

    IHC staining of mouse kidney using 17650-1-AP (same clone as 17650-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17650-1-AP (PAAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17650-1-PBS in a different storage buffer formulation.

PAAF1 Polyclonal antibody, PBS Only

PAAF1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17650-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

WDR71, WD repeat-containing protein 71, Rpn14, Protein G-16, Proteasomal ATPase-associated factor 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17650-1-PBS targets PAAF1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11892

Product name: Recombinant human PAAF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-375 aa of BC028628

Sequence: MLVPCFLYSLQNRKPSLYGSLTCQGIGLDGIPEVTASEGFTVNEINKKSIHISCPKENASSKFLAPYTTFSRIHTKSITCLDISSRGGLGVSSSTDGTMKIWQASNGELRRVLEGHVFDVNCCRFFPSGLVVLSGGMDAQLKIWSAEDASCVVTFKGHKGGILDTAIVDRGRNVVSASRDGTARLWDCGRSACLGVLADCGSSINGVAVGAADNSINLGSPEQMPSEREVGTEAKMLLLAREDKKLQCLGLQSRQLVFLFIGSDAFNCCTFLSGFLLLAGTQDGNIYQLDVRSPRAPVQVIHRSGAPVLSLLSVRDGFIASQGDGSCFIVQQDLDYVTELTGADCDPVYKVATWEKQIYTCCRDGLVRRYQLSDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasomal ATPase-associated factor 1
别名 WDR71, WD repeat-containing protein 71, Rpn14, Protein G-16, Proteasomal ATPase-associated factor 1
计算分子量 375 aa, 40 kDa
观测分子量 30 kDa
GenBank蛋白编号BC028628
基因名称 PAAF1
Gene ID (NCBI) 80227
RRIDAB_2878424
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRP4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAAF1(Proteasomal ATPase-associated factor 1) is also named as WDR71 and belongs to the WD repeat PAAF1/RPN14 family. It functions as a negative regulator of the proteasome by controlling the assembly/disassembly of the proteasome. PAAF1 has 3 isoforms produced by alternative splicing.

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