验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HEK-293 cells, HepG2 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
| IF | See 1 publications below |
产品信息
30092-1-AP targets P85a; PIK3R1 in WB, IF, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphoinositide-3-kinase, regulatory subunit 1 (alpha) |
| 别名 | GRB1, p85, p85 ALPHA, PI3 kinase subunit p85 alpha, PI3K regulatory subunit alpha, PIK3R1 |
| 计算分子量 | 85 kDa |
| 观测分子量 | 85 kDa |
| GenBank蛋白编号 | BC030815 |
| 基因名称 | PI3 Kinase p85 Alpha |
| Gene ID (NCBI) | 5295 |
| RRID | AB_3086227 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P27986 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P85a; PIK3R1 antibody 30092-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis The dual mechanism of m6A demethylase ALKBH5 in regulating energy metabolism during exposure to MC-LR | ||
Int J Biol Sci BRD7 Inhibited Immune Escape in Nasopharyngeal Carcinoma via Inhibiting PD-L1 Expression | ||
J Ethnopharmacol Cornus officinalis loganin attenuates acute lung injury in mice via regulating the PI3K/AKT/NLRP3 axis | ||
Biomater Adv Rehmannia-derived nanovesicles hijack the cell cycle to suppress oral squamous cell carcinoma via PI3K-AKT pathway blockade. | ||
Int Immunopharmacol Vitamin D against diabetic adipose tissue inflammation through SHP-1/STAT3 pathway | ||
Front Pharmacol Immune-related gene risk model establishment and role of key gene FUCA1 in malignant pleural mesothelioma |
