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  • Featured Product
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P62,SQSTM1 Recombinant antibody

P62,SQSTM1 Recombinant Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D17

Cat No : 80294-1-RR

Print datasheet

Synonyms

A170, EBIAP, ORCA, OSIL, p60, P62, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3

验证数据展示

  • WB analysis using 80294-1-RR

    WB analysis using 80294-1-RR

    Lysates of HepG2 cells treated with Chloroquine or not were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 80294-1-RR

    WB analysis of HEK-293 using 80294-1-RR

    WB result of P62,SQSTM1 antibody (80294-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62,SQSTM1 transfected HEK-293 cells.

  • WB analysis using 80294-1-RR

    WB analysis using 80294-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 80294-1-RR

    IF Staining of HeLa using 80294-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using P62,SQSTM1 antibody (80294-1-RR, Clone: 1D17 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IP experiment of HEK-293 using 80294-1-RR

    IP experiment of HEK-293 using 80294-1-RR

    IP result of anti-P62,SQSTM1 (IP:80294-1-RR, 4ug; Detection:80294-1-RR 1:500) with HEK-293 cells lysate 1640 ug.

  • IHC staining of human pancreas cancer using 80294-1-RR

    IHC staining of human pancreas cancer using 80294-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Jurkat using 80294-1-RR

    FC experiment of Jurkat using 80294-1-RR

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human P62,SQSTM1 (80294-1-RR, Clone:1D17) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, MCF-7 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inChloroquine treated HeLa cells
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

80294-1-RR targets P62,SQSTM1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen P62,SQSTM1 fusion protein Ag13131 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sequestosome 1
Synonyms A170, EBIAP, ORCA, OSIL, p60, P62, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3
Calculated Molecular Weight 48 kDa
Observed Molecular Weight62 kDa
GenBank Accession NumberBC017222
Gene Symbol SQSTM1
Gene ID (NCBI) 8878
RRIDAB_2918883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13501
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease, and nephropathic cystinosis (PMID: 22074114, 22860231, 22714671).

实验方案

Product Specific Protocols
WB protocol for P62,SQSTM1 antibody 80294-1-RRDownload protocol
IHC protocol for P62,SQSTM1 antibody 80294-1-RRDownload protocol
IF protocol for P62,SQSTM1 antibody 80294-1-RRDownload protocol
IP protocol for P62,SQSTM1 antibody 80294-1-RRDownload protocol
FC protocol for P62,SQSTM1 antibody 80294-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Toxicol In Vitro

Paraquat-induced ferroptosis suppression via NRF2 expression regulation

Authors - Qiqi Cai
View Article