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验证数据展示

  • WB analysis using 68184-1-Ig (same clone as 68184-1-PBS)

    WB analysis using 68184-1-Ig (same clone as 68184-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68184-1-Ig (P5CS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68184-1-PBS in a different storage buffer formulation.

  • WB analysis of HAP1 using 68184-1-Ig (same clone as 68184-1-PBS)

    WB analysis of HAP1 using 68184-1-Ig (same clone as 68184-1-PBS)

    WB result of P5CS antibody (68184-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-P5CS transfected HAP1 cells. This data was developed using the same antibody clone with 68184-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

P5CS Monoclonal antibody, PBS Only

P5CS Monoclonal Antibody for WB, ELISA
Cat No. 68184-1-PBS

产品说明书

CloneNo. 3B1E11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

ALDH18A1, 3B1E11, Aldehyde dehydrogenase family 18 member A1, Delta-1-pyrroline-5-carboxylate synthase, Gamma-glutamyl kinase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68184-1-PBS targets P5CS in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12100

Product name: Recombinant human ALDH18A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-362 aa of BC106054

Sequence: MLSQVYRCGFQPFNQHLLPWVKCTTVFRSHCIQPSVIRHVRSWSNIPFITVPLSRTHGKSFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMAIPVLEARACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGDQQSVTFGIKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPT

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 aldehyde dehydrogenase 18 family, member A1
别名 ALDH18A1, 3B1E11, Aldehyde dehydrogenase family 18 member A1, Delta-1-pyrroline-5-carboxylate synthase, Gamma-glutamyl kinase
计算分子量 795 aa, 87 kDa
观测分子量 87 kDa
GenBank蛋白编号BC106054
基因名称 P5CS
Gene ID (NCBI) 5832
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP54886
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.