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  • KD/KO Validated

DDX6 Polyclonal antibody

DDX6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14632-1-AP

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Synonyms

RCK, Probable ATP-dependent RNA helicase DDX6, P54, HLR2, EC:3.6.4.13



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, C2C12 cells, mouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, hTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14632-1-AP targets DDX6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen DDX6 fusion protein Ag6211 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
Synonyms RCK, Probable ATP-dependent RNA helicase DDX6, P54, HLR2, EC:3.6.4.13
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC065007
Gene Symbol DDX6
Gene ID (NCBI) 1656
RRIDAB_2091264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26196
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P54, also named as DDX6, HLR2, and RCK, is a 483 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. P54 is abundantly expressed in most tissues. In the process of mRNA degradation, P54 may play a role in mRNA decapping. P54 is involved in the replication of hepatitis C virus genomes in hepatocytes and in tumourigenesis of hepatocellular carcinomas.

实验方案

Product Specific Protocols
WB protocol for DDX6 antibody 14632-1-APDownload protocol
IHC protocol for DDX6 antibody 14632-1-APDownload protocol
IF protocol for DDX6 antibody 14632-1-APDownload protocol
IP protocol for DDX6 antibody 14632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

J Genet Genomics

LSM14B coordinates protein component expression in the P-body and controls oocyte maturation

Authors - Huiru Zhang
humanWB,IF

iScience

The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex.

Authors - Limei Zou
mouseWB

Biol Reprod

Translational Activation of Developmental Messenger RNAs During Neonatal Mouse Testis Development.

Authors - Chappell Vesna A VA
humanWB

Biochem Biophys Res Commun

DDX6 is involved in the pathogenesis of inflammatory diseases via NF-κB activation

Authors - Seiichiro Naito
  • KD Validated
humanWB

EMBO J

Energy stress promotes P-bodies formation via lysine-63-linked polyubiquitination of HAX1

Authors - Wanqi Zhan
mouseIF

BMC Bioinformatics

SpotitPy: a semi-automated tool for object-based co-localization of fluorescent labels in microscopy images

Authors - Alexia Akalestou-Clocher