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验证数据展示

  • WB analysis using 85374-1-RR (same clone as 85374-1-PBS)

    WB analysis using 85374-1-RR (same clone as 85374-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85374-1-RR (P4HA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85374-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 85374-1-RR (same clone as 85374-1-PBS)

    IHC staining of human stomach cancer using 85374-1-RR (same clone as 85374-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85374-1-RR (P4HA3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85374-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 85374-1-RR (same clone as 85374-1-PBS)

    IF Staining of A549 using 85374-1-RR (same clone as 85374-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using P4HA3 antibody (85374-1-RR, Clone: 242750C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85374-1-PBS in a different storage buffer formulation.

  • Affinity of 85374-1-RR

    Affinity of 85374-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85374-1-RR against Human P4HA3 were performed. The affinity constant is below 1 pM.

P4HA3 Recombinant monoclonal antibody, PBS Only

P4HA3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85374-1-PBS

产品说明书

CloneNo. 242750C3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

4-PH alpha-3, EC:1.14.11.2, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3, Prolyl 4-hydroxylase subunit alpha-3, UNQ711/PRO1374

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85374-1-PBS targets P4HA3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19716

Product name: Recombinant human P4HA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-256 aa of BC126170

Sequence: GDTFSALTSVARALAPERRLLGLLRRYLRGEEARLRDLTRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGSAITDLYSPKRLFSLTGDDCFQVGKVAYDMGDYYHAIPWLEEAVSLFRGSYGEWKTEDEASLEDALDHLAFAYFRAGNVSCALSLSREFLLY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 prolyl 4-hydroxylase, alpha polypeptide III
别名 4-PH alpha-3, EC:1.14.11.2, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-3, Prolyl 4-hydroxylase subunit alpha-3, UNQ711/PRO1374
计算分子量 544 aa, 61 kDa
观测分子量61 kDa
GenBank蛋白编号BC126170
基因名称 P4HA3
Gene ID (NCBI) 283208
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z4N8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Collagen prolyl 4-hydroxylases catalyze the hydroxylation of proline in x-pro-gly triplets in collagens and in proteins with collagen-like sequences. Collagen prolyl 4-hydroxylases are tetramers of 2 alpha subunits, such as P4HA3, and 2 beta subunits(P4HB). P4HA3 has 3 isoforms produced by alternative splicing with the MW of 61 kDa, 29 kDa and 67 kDa.

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