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  • KD/KO Validated

P4HA3 Polyclonal antibody

P4HA3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 23185-1-AP

Print datasheet

Synonyms

4 PH alpha 3, P4HA3



经过测试的应用

Positive WB detected inmouse kidney tissue, A549 cells
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23185-1-AP targets P4HA3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen P4HA3 fusion protein Ag19716 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prolyl 4-hydroxylase, alpha polypeptide III
Synonyms 4 PH alpha 3, P4HA3
Calculated Molecular Weight 544 aa, 61 kDa
Observed Molecular Weight 30 kDa, 61 kDa
GenBank Accession NumberBC126170
Gene Symbol P4HA3
Gene ID (NCBI) 283208
RRIDAB_2879228
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z4N8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Collagen prolyl 4-hydroxylases catalyze the hydroxylation of proline in x-pro-gly triplets in collagens and in proteins with collagen-like sequences. Collagen prolyl 4-hydroxylases are tetramers of 2 alpha subunits, such as P4HA3, and 2 beta subunits(P4HB). P4HA3 has 3 isoforms produced by alternative splicing with the MW of 61 kDa, 29 kDa and 67 kDa.

实验方案

Product Specific Protocols
WB protocol for P4HA3 antibody 23185-1-APDownload protocol
IF protocol for P4HA3 antibody 23185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,ratWB

J Mol Cell Cardiol

Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion.

Authors - Randy T Cowling
humanWB

Commun Biol

TGF-β-dependent reprogramming of amino acid metabolism induces epithelial-mesenchymal transition in non-small cell lung cancers.

Authors - Fumie Nakasuka
  • KD Validated
mouseIHC

J Dent Sci

Thrombospondin-1 and prolyl 4-hydroxylase subunit alpha 3 as potential biomarkers of salivary gland fibrosis

Authors - Zijian Zhang
humanWB

bioRxiv

Identifying and targeting key driver genes for collagen production within the 11q13/14 breast cancer amplicon

Authors - Daniela Araiza-Olivera
  • KD Validated
humanIHC

Heliyon

Collagen synthase P4HA3 as a novel biomarker for colorectal cancer correlates with prognosis and immune infiltration

Authors - Xiaohuan Guo
humanWB

Mol Cell Biochem

Transcriptome sequencing identifies prognostic genes involved in gastric adenocarcinoma

Authors - Mingyue Li
  • KD Validated