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验证数据展示

  • WB analysis of HeLa using 66604-1-Ig (same clone as 66604-1-PBS)

    WB analysis of HeLa using 66604-1-Ig (same clone as 66604-1-PBS)

    WB result of P4HA2 antibody (66604-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P4HA2 transfected HeLa cells. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 66604-1-Ig (same clone as 66604-1-PBS)

    WB analysis of HSC-T6 using 66604-1-Ig (same clone as 66604-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P4HA2 antibody included
  • IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P4HA2 antibody included
  • Featured Product
  • KD/KO验证

P4HA2 Monoclonal antibody, PBS Only

P4HA2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66604-1-PBS

产品说明书

CloneNo. 3A9A4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

4 PH alpha 2, 4-PH alpha-2, EC:1.14.11.2, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2, Prolyl 4-hydroxylase subunit alpha-2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy P4HA2 Ready-To-Use IHC Kit. P4HA2 primary antibody included.

产品信息

66604-1-PBS targets P4HA2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 P4HA2 fusion protein Ag4714 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 prolyl 4-hydroxylase, alpha polypeptide II
别名 4 PH alpha 2, 4-PH alpha-2, EC:1.14.11.2, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2, Prolyl 4-hydroxylase subunit alpha-2
计算分子量 533 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC035813
基因名称 P4HA2
Gene ID (NCBI) 8974
RRIDAB_2881964
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15460
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. P4HA2 is one of at least 2 alpha subunit isoforms. It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This pprotein has 2 isoforms produced by alternative splicing.