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验证数据展示

  • WB analysis of HeLa using 66604-1-Ig (same clone as 66604-1-PBS)

    WB analysis of HeLa using 66604-1-Ig (same clone as 66604-1-PBS)

    WB result of P4HA2 antibody (66604-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P4HA2 transfected HeLa cells. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 66604-1-Ig (same clone as 66604-1-PBS)

    WB analysis of HSC-T6 using 66604-1-Ig (same clone as 66604-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66604-1-Ig (P4HA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P4HA2 antibody included
  • IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    IHC staining of human lung cancer using 66604-1-Ig (same clone as 66604-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66604-1-Ig (P4HA2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66604-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P4HA2 antibody included
  • Featured Product
  • KD/KO验证

P4HA2 Monoclonal antibody, PBS Only

P4HA2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66604-1-PBS

产品说明书

CloneNo. 3A9A4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

4 PH alpha 2, 4-PH alpha-2, EC:1.14.11.2, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2, Prolyl 4-hydroxylase subunit alpha-2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy P4HA2 Ready-To-Use IHC Kit. P4HA2 primary antibody included.

产品信息

66604-1-PBS targets P4HA2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4714

Product name: Recombinant human P4HA2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 184-533 aa of BC035813

Sequence: HTVLWMEQVLKQLDAGEEATTTKSQVLDYLSYAVFQLGDLHRALELTRRLLSLDPSHERAGGNLRYFEQLLEEEREKTLTNQTEAELATPEGIYERPVDYLPERDVYESLCRGEGVKLTPRRQKRLFCRYHHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHITGLTVKTAELLQVANYGVGGQYEPHFDFSRRPFDSGLKTEGNRLATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVSNKWFHERGQEFLRPCGSTEVD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 prolyl 4-hydroxylase, alpha polypeptide II
别名 4 PH alpha 2, 4-PH alpha-2, EC:1.14.11.2, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2, Prolyl 4-hydroxylase subunit alpha-2
计算分子量 533 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC035813
基因名称 P4HA2
Gene ID (NCBI) 8974
RRIDAB_2881964
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO15460
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Prolyl 4-hydroxylase catalyzes the posttranslational formation of 4-hydroxyproline in collagens. The vertebrate enzyme is a tetramer of 2 alpha subunits and 2 beta subunits. P4HA2 is one of at least 2 alpha subunit isoforms. It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. This pprotein has 2 isoforms produced by alternative splicing.

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