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P3H1 Polyclonal antibody

P3H1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 26691-1-AP

Print datasheet

Synonyms

GROS1, Growth suppressor 1, LEPRE1, Leprecan 1, P3H1, Prolyl 3 hydroxylase 1

验证数据展示

  • WB analysis of HeLa using 26691-1-AP

    WB analysis of HeLa using 26691-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26691-1-AP (P3H1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 26691-1-AP

    IHC staining of human testis using 26691-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26691-1-AP (P3H1 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 26691-1-AP

    IHC staining of human testis using 26691-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26691-1-AP (P3H1 antibody at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman testis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26691-1-AP is P3H1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen P3H1 fusion protein Ag24904 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine proline-enriched proteoglycan (leprecan) 1
Synonyms GROS1, Growth suppressor 1, LEPRE1, Leprecan 1, P3H1, Prolyl 3 hydroxylase 1
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC108311
Gene Symbol LEPRE1
Gene ID (NCBI) 64175
RRIDAB_2880605
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ32P28
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P3H1 (prolyl 3-hydroxylase 1, also known as LEPRE1) is a member of the leprecan family of proteins, which also include P3H2, P3H3, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H1 is localized to the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. Mutations in this gene are associated with osteogenesis imperfecta type VIII.

实验方案

Product Specific Protocols
WB protocol for P3H1 antibody 26691-1-APDownload protocol
IHC protocol for P3H1 antibody 26691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

J Oncol

The Prognostic Significance and Potential Mechanism of Prolyl 3-Hydroxylase 1 in Hepatocellular Carcinoma

Authors - Chunlei Li
View Article