验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, HepG2 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17569-1-AP targets P2RY8 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag11775 Product name: Recombinant human P2RY8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 189-359 aa of BC043610 Sequence: MWAVFLFTIFILLFLIPFVITVACYTATILKLLRTEEAHGREQRRRAVGLAAVVLLAFVTCFAPNNFVLLAHIVSRLFYGKSYYHVYKLTLCLSCLNNCLDPFVYYFASREFQLRLREYLGCRRVPRDTLDTRRESLFSARTTSVRSEAGAHPEGMEGATRPGLQRQESVF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | purinergic receptor P2Y, G-protein coupled, 8 |
| 别名 | P2Y8, P2Y purinoceptor 8 |
| 计算分子量 | 359 aa, 41 kDa |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | BC043610 |
| 基因名称 | P2RY8 |
| Gene ID (NCBI) | 286530 |
| RRID | AB_3669278 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86VZ1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
P2RY8 (P2Y purinoceptor 8) belongs to the family of G-protein coupled receptors and is a Gα13-coupled receptor that mediates migration inhibition and growth regulation of B cells in lymphoid tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P2RY8 antibody 17569-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
