验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, MCF-7 cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20297-1-AP targets P2RY2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | P2RY2 fusion protein Ag14107 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | purinergic receptor P2Y, G-protein coupled, 2 |
| 别名 | P2U receptor 1, P2U purinoceptor 1, P2U, P2RU1, HP2U |
| 计算分子量 | 377 aa, 42 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC012104 |
| 基因名称 | P2RY2 |
| Gene ID (NCBI) | 5029 |
| RRID | AB_3669332 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P41231 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
P2RY2, also named P2RU1, P2U1, and P2Y2, belongs to the G-protein coupled receptor 1 family. It is a receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. Extracellular ATP then acts on purinergic receptors, including P2RY2, to activate proline-rich tyrosine kinase 2 (Pyk2) kinase and transient plasma membrane depolarization.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P2RY2 antibody 20297-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |