验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20191-1-AP targets P2RY11 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | P2RY11 fusion protein Ag14106 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | purinergic receptor P2Y, G-protein coupled, 11 |
| 别名 | P2RY11, P2Y purinoceptor 11, P2Y11 |
| 计算分子量 | 374 aa, 40 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC009877 |
| 基因名称 | P2RY11 |
| Gene ID (NCBI) | 5032 |
| RRID | AB_3669328 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96G91 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2Y purinoceptor 11 (P2RY11) is a receptor for ATP and ADP coupled to G-proteins that activate both phosphatidylinositol-calcium and adenylyl cyclase second messenger systems. It is not activated by UTP or UDP (PMID: 9405388). Expression of P2RY11 has been detected in immune cells including dendritic cells, macrophages and non‐immune cells including cancer cells (PMID: 33463722). It is involved in immune regulation (PMID: 27246167). No murine P2ry11 has been identified.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P2RY11 antibody 20191-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |