验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse heart tissue |
| Positive IHC detected in | human testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18273-1-AP targets P2RY1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag12992 Product name: Recombinant human P2RY1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 281-373 aa of BC074785 Sequence: TMNLRARLDFQTPAMCAFNDRVYATYQVTRGLASLNSCVDPILYFLAGDTFRRRLSRATRKASRRSEANLQSKSEDMTLNILPEFKQNGDTSL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | purinergic receptor P2Y, G-protein coupled, 1 |
| 别名 | ATP receptor, P2RY1, P2Y purinoceptor 1, P2Y1, Purinergic receptor |
| 计算分子量 | 373 aa, 42 kDa |
| 观测分子量 | 42 kDa, 57 kDa-59 kDa, 66 kDa |
| GenBank蛋白编号 | BC074785 |
| 基因名称 | P2RY1 |
| Gene ID (NCBI) | 5028 |
| RRID | AB_2158402 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P47900 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for P2RY1 antibody 18273-1-AP | Download protocol |
| WB protocol for P2RY1 antibody 18273-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
