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P2RX5 Monoclonal antibody

P2RX5 Monoclonal Antibody for FC, IF-P, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

FC, IF-P, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A2A6

Cat No : 66430-1-Ig

Print datasheet

Synonyms

ATP receptor, P2RX5, P2X purinoceptor 5, P2X5, P2X5R, Purinergic receptor

验证数据展示

  • IF Staining of mouse brown adipose using 66430-1-Ig

    IF Staining of mouse brown adipose using 66430-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using P2RX5 antibody (66430-1-Ig, Clone: 2A2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis using 66430-1-Ig

    WB analysis using 66430-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66430-1-Ig (P2RX5 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 66430-1-Ig

    FC experiment of Jurkat using 66430-1-Ig

    1X10^6 Jurkat cells were stained with 0.20 ug Anti-Human P2RX5 (66430-1-Ig, Clone:2A2A6) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH.

经过测试的应用

Positive WB detected inHEK-293 cells, pig brain tissue, Jurkat cells, K-562 cells, rat brain tissue, mouse brain tissue
Positive IF detected inmouse brown adipose tissue
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66430-1-Ig targets P2RX5 in WB, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications FC, IF-P, WB, ELISA
Tested Reactivity human, mouse, rat, pig
Immunogen P2RX5 fusion protein Ag5297 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name purinergic receptor P2X, ligand-gated ion channel, 5
Synonyms ATP receptor, P2RX5, P2X purinoceptor 5, P2X5, P2X5R, Purinergic receptor
Calculated Molecular Weight 422 aa, 47 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC039015
Gene Symbol P2RX5
Gene ID (NCBI) 5026
RRIDAB_2881801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ93086
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for P2RX5 antibody 66430-1-IgDownload protocol
IF protocol for P2RX5 antibody 66430-1-IgDownload protocol
FC protocol for P2RX5 antibody 66430-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols