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验证数据展示

  • IF Staining of mouse brown adipose using 66430-1-Ig

    IF Staining of mouse brown adipose using 66430-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using P2RX5 antibody (66430-1-Ig, Clone: 2A2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 66430-1-Ig

    WB analysis using 66430-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66430-1-Ig (P2RX5 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

P2RX5 Monoclonal antibody

P2RX5 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 66430-1-Ig

产品说明书

CloneNo. 2A2A6

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IF-P, ELISA

别名

ATP receptor, P2RX5, P2X purinoceptor 5, P2X5, P2X5R, Purinergic receptor

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, pig brain tissue, Jurkat cells, K-562 cells, rat brain tissue, mouse brain tissue
Positive IF-P detected inmouse brown adipose tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66430-1-Ig targets P2RX5 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag5297

Product name: Recombinant human P2RX5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 52-422 aa of BC039015

Sequence: KKGYQDVDTSLQSAVITKVKGVAFTNTSDLGQRIWDVADYVIPAQGENVFFVVTNLIVTPNQRQNVCAENEGIPDGACSKDSDCHAGEAVTAGNGVKTGRCLRRENLARGTCEIFAWCPLETSSRPEEPFLKEAEDFTIFIKNHIRFPKFNFSKSNVMDVKDRSFLKSCHFGPKNHYCPIFRLGSVIRWAGSDFQDIALEGGVIGINIEWNCDLDKAASECHPHYSFSRLDNKLSKSVSSGYNFRFARYYRDAAGVEFRTLMKAYGIRFDVMVNGKGAFFCDLVLIYLIKKREFYRDKKYEEVRGLEDSSQEAEDEASGLGLSEQLTSGPGLLGMPEQQELQEPPEAKRGSSSQKGNGSVCPQLLEPHRST

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 purinergic receptor P2X, ligand-gated ion channel, 5
别名 ATP receptor, P2RX5, P2X purinoceptor 5, P2X5, P2X5R, Purinergic receptor
计算分子量 422 aa, 47 kDa
观测分子量 52 kDa
GenBank蛋白编号BC039015
基因名称 P2RX5
Gene ID (NCBI) 5026
RRIDAB_2881801
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ93086
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for P2RX5 antibody 66430-1-IgDownload protocol
IF protocol for P2RX5 antibody 66430-1-IgDownload protocol
WB protocol for P2RX5 antibody 66430-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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