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P2RX5 Polyclonal antibody

P2RX5 Polyclonal Antibody for FC, IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 19012-1-AP

Print datasheet

Synonyms

ATP receptor, P2RX5, P2X purinoceptor 5, P2X5, P2X5R, Purinergic receptor

验证数据展示

  • WB analysis of BxPC-3 using 19012-1-AP

    WB analysis of BxPC-3 using 19012-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 19012-1-AP (P2RX5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 19012-1-AP

    IF Staining of BxPC-3 using 19012-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed BxPC-3 cells using 19012-1-AP (P2RX5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of K-562 using 19012-1-AP

    FC experiment of K-562 using 19012-1-AP

    1X10^6 K-562 cells were stained with 0.2ug P2RX5 antibody (19012-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inBxPC-3 cells, HL-60 cells, MDA-MB-453s cells
Positive IF detected inBxPC-3 cells
Positive FC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

19012-1-AP targets P2RX5 in WB, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC, WB,ELISA
Cited ApplicationsFC
Tested Reactivity human
Cited Reactivityhuman
Immunogen P2RX5 fusion protein Ag5297 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name purinergic receptor P2X, ligand-gated ion channel, 5
Synonyms ATP receptor, P2RX5, P2X purinoceptor 5, P2X5, P2X5R, Purinergic receptor
Calculated Molecular Weight 422 aa, 47 kDa
Observed Molecular Weight 44 kDa, 49 kDa, 116 kDa
GenBank Accession NumberBC039015
Gene Symbol P2RX5
Gene ID (NCBI) 5026
RRIDAB_2299079
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ93086
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for P2RX5 antibody 19012-1-APDownload protocol
IF protocol for P2RX5 antibody 19012-1-APDownload protocol
FC protocol for P2RX5 antibody 19012-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanFC

PLoS One

A Truncation Variant of the Cation Channel P2RX5 Is Upregulated during T Cell Activation.

Authors - Pierre Abramowski
View Article