×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 25614-1-AP (same clone as 25614-1-PBS)

    WB analysis using 25614-1-AP (same clone as 25614-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25614-1-AP (P27; KIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25614-1-PBS in a different storage buffer formulation.

  • WB analysis using 25614-1-AP (same clone as 25614-1-PBS)

    WB analysis using 25614-1-AP (same clone as 25614-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25614-1-AP (P27; KIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25614-1-PBS in a different storage buffer formulation.

  • WB analysis using 25614-1-AP (same clone as 25614-1-PBS)

    WB analysis using 25614-1-AP (same clone as 25614-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25614-1-AP (P27; KIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25614-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 25614-1-AP (same clone as 25614-1-PBS)

    WB analysis of HeLa using 25614-1-AP (same clone as 25614-1-PBS)

    WB result of P27; KIP1 antibody (25614-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P27; KIP1 transfected HeLa cells. This data was developed using the same antibody clone with 25614-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 25614-1-AP (same clone as 25614-1-PBS)

    IHC staining of human breast cancer using 25614-1-AP (same clone as 25614-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25614-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25614-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDKN1B antibody included
  • IHC staining of human breast cancer using 25614-1-AP (same clone as 25614-1-PBS)

    IHC staining of human breast cancer using 25614-1-AP (same clone as 25614-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25614-1-AP (P27; KIP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25614-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDKN1B antibody included
  • IF Staining of mouse testis using 25614-1-AP (same clone as 25614-1-PBS)

    IF Staining of mouse testis using 25614-1-AP (same clone as 25614-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using P27; KIP1 antibody (25614-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25614-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 25614-1-AP (same clone as 25614-1-PBS)

    IF Staining of mouse testis using 25614-1-AP (same clone as 25614-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using P27; KIP1 antibody (25614-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25614-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 25614-1-AP (same clone as 25614-1-PBS)

    FC experiment of MCF-7 using 25614-1-AP (same clone as 25614-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human P27; KIP1 (25614-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 25614-1-PBS in a different storage buffer formulation.

  • IP experiment of NIH/3T3 using 25614-1-AP (same clone as 25614-1-PBS)

    IP experiment of NIH/3T3 using 25614-1-AP (same clone as 25614-1-PBS)

    IP result of anti-P27; KIP1 (IP:25614-1-AP, 4ug; Detection:25614-1-AP 1:1000) with NIH/3T3 cells lysate 1160 ug. This data was developed using the same antibody clone with 25614-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 25614-1-AP (same clone as 25614-1-PBS)

    IF Staining of HepG2 using 25614-1-AP (same clone as 25614-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P27; KIP1 antibody (25614-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 25614-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

P27; KIP1 Polyclonal antibody, PBS Only

P27; KIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA
Cat No. 25614-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

别名

CDKN1B, P27, CDKN4, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy CDKN1B Ready-To-Use IHC Kit. CDKN1B primary antibody included.

产品信息

25614-1-PBS targets P27; KIP1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22582

Product name: Recombinant human P27; KIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 44-198 aa of BC001971

Sequence: DLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDGSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 1B (p27, Kip1)
别名 CDKN1B, P27, CDKN4, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27
计算分子量 198 aa, 22 kDa
观测分子量 27 kDa
GenBank蛋白编号BC001971
基因名称 p27 Kip1/CDKN1B
Gene ID (NCBI) 1027
RRIDAB_2880161
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46527
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.

||
New chat

Able™

正在加载，请稍候...