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- KD/KO Validated
P27; KIP1 Polyclonal antibody
P27; KIP1 Polyclonal Antibody for FC, IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC, IF, IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | NIH/3T3 cells, MCF-7 cells, HeLa cells |
Positive IHC detected in | human gliomas tissue, human tonsillitis tissue, human lung cancer tissue, human breast cancer tissue, human colon cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Positive FC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
发表文章中的应用
WB | See 2 publications below |
产品信息
The immunogen of 26714-1-AP is P27; KIP1 Fusion Protein expressed in E. coli.
Tested Applications | FC, IF, IHC, WB,ELISA |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | P27; KIP1 fusion protein Ag25083 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cyclin-dependent kinase inhibitor 1B (p27, Kip1) |
Synonyms | CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27, P27; KIP1, P27KIP1 |
Calculated molecular weight | 198 aa, 22 kDa |
Observed molecular weight | 27 kDa |
GenBank accession number | BC001971 |
Gene symbol | CDKN1B |
Uniprot | P46527 |
Gene ID (NCBI) | 1027 |
RRID | AB_2880611 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.
实验方案
Product Specific Protocols | |
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WB protocol for P27; KIP1 antibody 26714-1-AP | Download protocol |
IHC protocol for P27; KIP1 antibody 26714-1-AP | Download protocol |
IF protocol for P27; KIP1 antibody 26714-1-AP | Download protocol |
FC protocol for P27; KIP1 antibody 26714-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biosci Rep C1QTNF6 regulates cell proliferation and apoptosis of NSCLC in vitro and in vivo. | ||
Int J Oncol Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells. |