• Featured Product
  • KD/KO Validated

P21 Polyclonal antibody

P21 Polyclonal Antibody for WB, IP, IF/ICC, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (4)


WB, IP, IF/ICC, FC, IHC, ELISA and More (1)



Cat No : 10355-1-AP

Print datasheet


CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1


Positive WB detected inHUVEC cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, THP-1 cells, LNCaP cells, NCCIT cells, U2OS cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman cervical cancer tissue, human breast cancer tissue, human colon cancer tissue, human thyroid cancer tissue, human stomach cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, myotome of a somite at E11.5 tissue
Positive FC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.


Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


10355-1-AP targets P21 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IF/ICC, FC, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF, FC, IHC, CoIP
Tested Reactivity human
Cited Reactivityhuman, rabbit, pig, Artemia sinica, canine
Immunogen P21 fusion protein Ag0368 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Synonyms CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, p21CIP1, PIC1, SDI1, WAF1
Calculated Molecular Weight18 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC000275
Gene Symbol CDKN1A
Gene ID (NCBI) 1026
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.


Product Specific Protocols
WB protocol for P21 antibody 10355-1-APDownload protocol
IHC protocol for P21 antibody 10355-1-APDownload protocol
IF protocol for P21 antibody 10355-1-APDownload protocol
IP protocol for P21 antibody 10355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.

Authors - Yuhong Luo

Sci Transl Med

Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer

Authors - Jia-Han Ding

Nat Commun

Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity

Authors - Jinjie Duan

Nat Commun

URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers

Authors - Zhiwen Ding

Nat Commun

Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence

Authors - Yinsheng Wu

Mol Cancer

CircVAPA promotes small cell lung cancer progression by modulating the miR-377-3p and miR-494-3p/IGF1R/AKT axis.

Authors - Jinghan Hua