验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10272-2-AP targets p19 INK4d in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0415 Product name: Recombinant human P19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC001822 Sequence: MLLEEVRAGDRLSGAAARGDVQEVRRLLHRELVHPDALNRFGKTALQVMMFGSTAIALELLKQGASPNVQDTSGTSPVHDAARTGFLDTLKVLVEHGADVNVPDGTGALPIHLAVQEGHTAVVSFLAAESDLHRRDARGLTPLELALQRGAQDLVDILQGHMVAPL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) |
| 别名 | CDKN2D, INK4D, P19, p19 INK4D |
| 计算分子量 | 19 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC001822 |
| 基因名称 | p19 INK4d/CDKN2D |
| Gene ID (NCBI) | 1032 |
| RRID | AB_2078742 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55273 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for p19 INK4d antibody 10272-2-AP | Download protocol |
| IHC protocol for p19 INK4d antibody 10272-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Blood Interconnecting molecular pathways in the pathogenesis and drug sensitivity of T-cell acute lymphoblastic leukemia. | ||
Oxid Med Cell Longev Resveratrol Inhibits Ischemia-Induced Myocardial Senescence Signals and NLRP3 Inflammasome Activation. | ||
FASEB J Overexpression of ERBB4 rejuvenates aged mesenchymal stem cell and enhances angiogenesis via PI3K/AKT and MAPK/ERK pathway. | ||
Front Immunol Possible Roles of Proinflammatory Signaling in Keratinocytes Through Aryl Hydrocarbon Receptor Ligands for the Development of Squamous Cell Carcinoma. | ||
J Virol Rb-E2F-HDAC Repressor Complexes Control Interferon-Induced Repression of Adenovirus To Promote Persistent Infection. | ||
Lab Invest Th17/IL-17 induces endothelial cell senescence via activation of NF-κB/p53/Rb signaling pathway. |