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验证数据展示

  • WB analysis using 81373-8-RR

    WB analysis using 81373-8-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81373-8-RR (P16-INK4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 81373-8-RR

    WB analysis of HEK-293 using 81373-8-RR

    WB result of CDKN2A/P16-INK4A antibody (81373-8-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDKN2A/P16-INK4A transfected HEK-293 cells.

  • IHC staining of human cervical cancer using 81373-8-RR

    IHC staining of human cervical cancer using 81373-8-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81373-8-RR (P16-INK4A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDKN2A/P16INK4A antibody included
  • IHC staining of human tonsillitis using 81373-8-RR

    IHC staining of human tonsillitis using 81373-8-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81373-8-RR (P16-INK4A antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDKN2A/P16INK4A antibody included
  • IF Staining of HeLa using 81373-8-RR

    IF Staining of HeLa using 81373-8-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDKN2A/P16-INK4A antibody (81373-8-RR, Clone: 241438C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 81373-8-RR

    Affinity and Kinetic Characterization of 81373-8-RR

    Biolayer interferometry (BLl) kinetic assays of 81373-8-RR against Human P16-INK4A were performed. The affinity constant is 0.336 nM.

  • Featured Product
  • KD/KO验证

CDKN2A/P16-INK4A Recombinant monoclonal antibody

CDKN2A/P16-INK4A Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 81373-8-RR

产品说明书

CloneNo. 241438C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

p16, p16 INK4, P16 INK4A, p16-INK4, p16INK4A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 555
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, TF-1 cells, SiHa cells
Positive IHC detected inhuman cervical cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDKN2A/P16INK4A Ready-To-Use IHC Kit. CDKN2A/P16INK4A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81373-8-RR targets CDKN2A/P16-INK4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1328

Product name: Recombinant human P16-INK4A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 52-156 aa of BC021998

Sequence: MMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEELGHRDVARYLRAAAGGTRGSNHARIDAAEGPSDIPD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 2A
别名 p16, p16 INK4, P16 INK4A, p16-INK4, p16INK4A
计算分子量 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC021998
基因名称 CDKN2A
Gene ID (NCBI) 1029
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP42771
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively-spliced variants encoding distinct proteins proteins were reported. Two of them named p16-INK4 and p14 are sharing 50% identity. P16 plays an essential role in regulating the cell cycle, and mutations in p16 increase the risk of developing various cancers, including melanoma. Long-term p16(INK4a) expression pushes cells to enter senescence, an irreversible cell-cycle arrest that precludes the growth of would-be cancer cells but also contributes to cellular aging (PMID: 24136988).

实验方案

Product Specific Protocols
IF protocol for CDKN2A/P16-INK4A antibody 81373-8-RRDownload protocol
IHC protocol for CDKN2A/P16-INK4A antibody 81373-8-RRDownload protocol
WB protocol for CDKN2A/P16-INK4A antibody 81373-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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