P16-INK4A Polyclonal antibody
P16-INK4A Polyclonal Antibody for FC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
FC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells |
Positive IP detected in | HEK-293 cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30519-1-AP targets P16-INK4A in WB, IP, FC, ELISA applications and shows reactivity with Human samples.
Tested Applications | FC, IP, WB, ELISA |
Tested Reactivity | Human |
Immunogen | P16-INK4A fusion protein Ag29567 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) |
Synonyms | ARF, ARF,CDKN2A, CDK4I, CDKN2, CDKN2A, CMM2, INK4, INK4a, MLM, MTS 1, MTS1, Multiple tumor suppressor 1, p14, p14ARF, P16, p16 INK4, p16 INK4a, p16INK4, p16INK4a, P19, TP16 |
Calculated Molecular Weight | 17 kDa |
Observed Molecular Weight | 16 kDa |
GenBank Accession Number | NM_000077 |
Gene Symbol | CDKN2A |
Gene ID (NCBI) | 1029 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8N726 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for P16-INK4A antibody 30519-1-AP | Download protocol |
IP protocol for P16-INK4A antibody 30519-1-AP | Download protocol |
FC protocol for P16-INK4A antibody 30519-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |