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验证数据展示

  • WB analysis using 87016-1-RR (same clone as 87016-1-PBS)

    WB analysis using 87016-1-RR (same clone as 87016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87016-1-RR (P130 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87016-1-PBS in a different storage buffer formulation.

  • WB analysis of rat brain using 87016-1-RR (same clone as 87016-1-PBS)

    WB analysis of rat brain using 87016-1-RR (same clone as 87016-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 87016-1-RR (P130 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87016-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 87016-1-RR (same clone as 87016-1-PBS)

    IF Staining of HepG2 using 87016-1-RR (same clone as 87016-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P130 antibody (87016-1-RR, Clone: 252136E4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87016-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 87016-1-RR

    Affinity and Kinetic Characterization of 87016-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87016-1-RR against Human P130 were performed. The affinity constant is 2.70 nM.

P130 Recombinant monoclonal antibody, PBS Only

P130 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87016-1-PBS

产品说明书

CloneNo. 252136E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

NOLC1, HCV NS5A-transactivated protein 13, Hepatitis C virus NS5A-transactivated protein 13, KIAA0035, NOPP130

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NOLC1 Ready-To-Use IHC Kit. NOLC1 primary antibody included.

产品信息

87016-1-PBS targets P130 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2382

Product name: Recombinant human P130 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 69-418 aa of BC006769

Sequence: TKPPPAKKAAESSSDSSDSDSSEDDEAPSKPAGTTKNSSNKPAVTTKSPAVKPAAAPKQPVGGGQKLLTRKADSSSSEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSRDSSSDSDSSSSEEEEEKTSKSAVKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKGSPRPQAPKANGTSALTAQNGKAAKNSEEEEEEKKKAAVVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFDSE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nucleolar and coiled-body phosphoprotein 1
别名 NOLC1, HCV NS5A-transactivated protein 13, Hepatitis C virus NS5A-transactivated protein 13, KIAA0035, NOPP130
计算分子量 73 kDa
观测分子量120-130 kDa
GenBank蛋白编号BC006769
基因名称 NOLC1
Gene ID (NCBI) 9221
RRIDAB_3745276
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14978
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nucleolar and coiled-body phosphoprotein 1 (NOLC1) is a phosphoprotein that transiently associates with the mature nucleolar H/ACA and C/D box small nucleolar ribonucleoproteins (snoRNPs), guiding site-specific 2'-O-meth-ylation and pseudouridylation of pre-rRNAs [PMID:21266110]. It contains a nuclear localization signal binding sequence and is thought to shuttle between the nucleolus and the cytoplasm. NOLC1 plays an essential role in the synthesis of rRNA and the biosynthesis of ribosomes [PMID:8972203].

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