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验证数据展示

  • WB analysis of NIH/3T3 using 12755-1-AP (same clone as 12755-1-PBS)

    WB analysis of NIH/3T3 using 12755-1-AP (same clone as 12755-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12755-1-AP (Osteoglycin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 12755-1-AP (same clone as 12755-1-PBS)

    IHC staining of human lung using 12755-1-AP (same clone as 12755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OGN antibody included
  • IHC staining of human lung using 12755-1-AP (same clone as 12755-1-PBS)

    IHC staining of human lung using 12755-1-AP (same clone as 12755-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 12755-1-AP (Osteoglycin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OGN antibody included
  • IF Staining of HepG2 using 12755-1-AP (same clone as 12755-1-PBS)

    IF Staining of HepG2 using 12755-1-AP (same clone as 12755-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Osteoglycin antibody (12755-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12755-1-PBS in a different storage buffer formulation.

Osteoglycin Polyclonal antibody, PBS Only

Osteoglycin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12755-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy OGN Ready-To-Use IHC Kit. OGN primary antibody included.

产品信息

12755-1-PBS targets Osteoglycin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3484

Product name: Recombinant human OGN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-298 aa of BC037273

Sequence: MKTLQSTLLLLLLVPLIKPAPPTQQDSRIIYDYGTDNFEESIFSQDYEDKYLDGKNIKEKETVIIPNEKSLQLQKDEAITPLPPKKENDEMPTCLLCVCLSGSVYCEEVDIDAVPPLPKESAYLYARFNKIKKLTAKDFADIPNLRRLDFTGNLIEDIEDGTFSKLSLLEELSLAENQLLKLPVLPPKLTLFNAKYNKIKSRGIKANAFKKLNNLTFLYLDHNALESVPLNLPESLRVIHLQFNNIASITDDTFCKANDTSYIRDRIEEIRLEGNPIVLGKHPNSFICLKRLPIGSYF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 osteoglycin
别名
计算分子量 298 aa, 34 kDa
观测分子量 34-40 kDa
GenBank蛋白编号BC037273
基因名称 OGN
Gene ID (NCBI) 4969
RRIDAB_10646479
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20774
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded protein induces ectopic bone formation in conjunction with transforming growth factor beta and may regulate osteoblast differentiation. High expression of the encoded protein may be associated with elevated heart left ventricular mass. Alternative splicing results in multiple transcript variants which forms proteins with different molecular weight.

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