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ORAI1 Polyclonal antibody

ORAI1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 14443-1-AP

Print datasheet

Synonyms

ORAI1, ORAI1 L1, ORAI1 L2, Orail L2, TMEM142A

验证数据展示

  • WB analysis of human spleen using 14443-1-AP

    WB analysis of human spleen using 14443-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 14443-1-AP (Orai1-Alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 14443-1-AP

    IHC staining of human colon cancer using 14443-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14443-1-AP (Orai1-Alpha antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 14443-1-AP

    IHC staining of human colon cancer using 14443-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14443-1-AP (Orai1-Alpha antibody at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman spleen tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14443-1-AP targets ORAI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ORAI calcium release-activated calcium modulator 1
Synonyms ORAI1, ORAI1 L1, ORAI1 L2, Orail L2, TMEM142A
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberNM_032790
Gene Symbol ORAI1
Gene ID (NCBI) 84876
RRIDAB_10639506
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96D31
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 35-45 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene. This antibody may be used to block the ion currents mediated by Orai1. ORAI1 contains 2 isoforms  produced by alternative initiation(uniprot: Q96D31). Catalog#14443-1-AP only recognises alpha isoform.

实验方案

Product Specific Protocols
WB protocol for ORAI1 antibody 14443-1-APDownload protocol
IHC protocol for ORAI1 antibody 14443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

PLoS One

Coupling of store-operated calcium entry to vasoconstriction is acid-sensing ion channel 1a dependent in pulmonary but not mesenteric arteries.

Authors - Selina M Garcia
View Article