验证数据展示
经过测试的应用
| Positive WB detected in | human spleen tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
14443-1-AP targets ORAI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ORAI calcium release-activated calcium modulator 1 |
| 别名 | ORAI1-L2, Orail-L2, Calcium release-activated calcium channel protein 1, CRACM1, ORAI1 L1 |
| 计算分子量 | 35 kDa |
| 观测分子量 | 44 kDa |
| GenBank蛋白编号 | NM_032790 |
| 基因名称 | ORAI1 |
| Gene ID (NCBI) | 84876 |
| RRID | AB_10639506 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96D31 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Store-operated calcium channels (SOCs) play essential functions in various physiological processes. Orai1 (also named CRACM1) is a critical molecular component of SOCs. Orai1 protein is a 35-45 kDa plasma membrane protein with 4 transmembrane domains. Human patients with a severe combinaed immunodeficiency (SCID) syndrome possess point mutations in Orai1 gene. This antibody may be used to block the ion currents mediated by Orai1. ORAI1 contains 2 isoforms produced by alternative initiation(uniprot: Q96D31). Catalog#14443-1-AP only recognises alpha isoform.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ORAI1 antibody 14443-1-AP | Download protocol |
| WB protocol for ORAI1 antibody 14443-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
