验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, L02 cells |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | mouse placenta tissue, human breast cancer tissue, human hysteromyoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23045-1-AP targets OXTR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag19074 Product name: Recombinant human OXTR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 327-389 aa of BC137443 Sequence: WIYMLFTGHLFHELVQRFLCCSASYLKGRRLGETSASKKSNSSSFVLSHRSSSQRSCSQPSTA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | OXTR |
| 别名 | OT R, OT-R, Oxytocin receptor |
| 计算分子量 | 389 aa, 43 kDa |
| 观测分子量 | 46 kDa, 67 kDa |
| GenBank蛋白编号 | BC137443 |
| 基因名称 | OXTR |
| Gene ID (NCBI) | 5021 |
| RRID | AB_2827425 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30559 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Oxytocin (OXT) is a neurohypophysial hormone that plays a role in lactation and parturition and in the central nervous system as a neurotransmitter involved in sex and maternal behavior (PMID: 1852313; 10811917). OXT acts through its receptor, OXTR, which belongs to the G protein-coupled 7-transmembrane receptor family. OXTR is expressed in the endometrium, myometrium, and mammary gland (PMID: 1313946). This antibody is raised against the C-terminal region of human OXTR. Two immunoreactive OXTR bands were found in the fat tissue, an unglycosylated 43 kDa form and a mature glycosylated 67 kDa form (PMID:32819381).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for OXTR antibody 23045-1-AP | Download protocol |
| IHC protocol for OXTR antibody 23045-1-AP | Download protocol |
| IP protocol for OXTR antibody 23045-1-AP | Download protocol |
| WB protocol for OXTR antibody 23045-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Physiol (Oxf) Oxytocin modulates neural processing of mitral/tufted cells in the olfactory bulb. | ||
Antioxidants (Basel) ΔMST and the Regulation of Cardiac CSE and OTR Expression in Trauma and Hemorrhage. | ||
Biochem Pharmacol Synergistic antitumor activity of 5-fluorouracil and atosiban against microsatellite stable colorectal cancer through restoring GATA3.
| ||
Front Pharmacol The Role of Formyl Peptide Receptor 1 in Uterine Contraction During Parturition. |
