验证数据展示
经过测试的应用
| Positive IP detected in | mouse pituitary gland tissue |
| Positive IHC detected in | human pituitary tissue, mouse ovary tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
18041-1-AP targets Oxytocin-neurophysin 1 in IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, IP, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag12672 Product name: Recombinant human OXT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 19-125 aa of BC101841 Sequence: ACYIQNCPLGGKRAAPDLDVRKCLPCGPGGKGRCFGPNICCAEELGCFVGTAEALRCQEENYLPSPCQSGQKACGSGGRCAVLGLCCSPDGCHADPACDAEATFSQR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | oxytocin, prepropeptide |
| 别名 | OXT, Neurophysin 1, Ocytocin Neurophysin 1, Oxytocin, Oxytocin neurophysin 1 |
| 计算分子量 | 125 aa, 13 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC101841 |
| 基因名称 | OXT |
| Gene ID (NCBI) | 5020 |
| RRID | AB_2918056 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01178 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Oxytocin-neurophysin 1, also called the oxytocin/neurophysin I prepropeptide, is produced in the hypothalamus as a single, inactive precursor that contains two parts: the nonapeptide hormone oxytocin and its carrier protein neurophysin I. After synthesis in magnocellular neurons of the supra-optic and paraventricular nuclei, the precursor is packaged into neurosecretory vesicles and transported down the axon to the posterior pituitary (neurohypophysis). During this axonal transport the precursor is proteolytically cleaved, generating mature oxytocin and its tightly bound neurophysin I; the complex is stored in nerve terminals and secreted into the systemic circulation upon appropriate stimulation.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Oxytocin-neurophysin 1 antibody 18041-1-AP | Download protocol |
| IP protocol for Oxytocin-neurophysin 1 antibody 18041-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
