• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

OXER1 Polyclonal antibody

OXER1 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 55202-1-AP

Print datasheet

Synonyms

G protein coupled receptor 170, GPCR, GPR170, OXER1, oxoeicosanoid (OXE) receptor 1, Oxoeicosanoid receptor 1, TG1019

验证数据展示

  • WB analysis of K-562 using 55202-1-AP

    WB analysis of K-562 using 55202-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 55202-1-AP (OXER1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 55202-1-AP

    IHC staining of human kidney using 55202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 55202-1-AP

    IHC staining of human kidney using 55202-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55202-1-AP (OXER1 antibody) at dilution of 1:50 (under 10x lens).

  • FC experiment of K-562 using 55202-1-AP

    FC experiment of K-562 using 55202-1-AP

    1X10^6 K-562 cells were stained with 0.2ug OXER1 antibody (55202-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55202-1-AP targets OXER1 in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, WB,ELISA
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name oxoeicosanoid (OXE) receptor 1
Synonyms G protein coupled receptor 170, GPCR, GPR170, OXER1, oxoeicosanoid (OXE) receptor 1, Oxoeicosanoid receptor 1, TG1019
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 43-46 kDa
GenBank Accession NumberNM_148962
Gene Symbol OXER1
Gene ID (NCBI) 165140
RRIDAB_10858396
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDS5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

OXER1, also named as GPR170 and TG1019, is a receptor for eicosanoids and polyunsaturated fatty acids such as 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-OXO-ETE), 5(S)-hydroperoxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5(S)-HPETE) and arachidonic acid. It seems to be coupled to the G(i)/G(o), families of heteromeric G proteins. (PMID:12065583) This antibody is specific to OXER1.

实验方案

Product Specific Protocols
WB protocol for OXER1 antibody 55202-1-APDownload protocol
IHC protocol for OXER1 antibody 55202-1-APDownload protocol
FC protocol for OXER1 antibody 55202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols