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验证数据展示

  • WB analysis of mouse testis using 17122-1-AP

    WB analysis of mouse testis using 17122-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17122-1-AP (OXCT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17122-1-AP

    IHC staining of human testis using 17122-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17122-1-AP (OXCT2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 17122-1-AP

    IHC staining of human testis using 17122-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17122-1-AP (OXCT2 antibody) at dilution of 1:100 (under 40x lens).

OXCT2 Polyclonal antibody

OXCT2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17122-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

3 oxoacid CoA transferase 2, 3 oxoacid CoA transferase 2A, FKSG25, OXCT2, SCOT T

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17122-1-AP targets OXCT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag10810

Product name: Recombinant human OXCT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-353 aa of BC030015

Sequence: YGTLVQEGGAPIRYTPDGHLALMSQPREVREFNGDHFLLERAIRADFALVKGWKADRAGNVVFRRSARNFNVPMCKAADVTAVEVEEIVEVGAFPPEDIHVPNIYVDRVIKGQKYEKRIERLTILKEEDGDAGKEEDARTRIIRRAALEFEDGMYANLGIGIPLLASNFISPSMTVHLHSENGILGLGPFPTEDEVDADLINAGKQTVTVLPGGCFFASDDSFAMIRGGHIQLTMLGAMQVSKYGDLANWMIPGKKVKGMGGAMDLVSSQKTRVVVTMQHCTKDNTPKIMEKCTMPLTGKRCVDRIITEKAVFDVHRKKELTLRELWEGLTVDDIKKSTGCAFAVSPNLRPMQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 3-oxoacid CoA transferase 2
别名 3 oxoacid CoA transferase 2, 3 oxoacid CoA transferase 2A, FKSG25, OXCT2, SCOT T
计算分子量 517 aa, 56 kDa
观测分子量 55 kDa
GenBank蛋白编号BC030015
基因名称 OXCT2
Gene ID (NCBI) 64064
RRIDAB_2157447
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYC2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OXCT2 antibody 17122-1-APDownload protocol
IHC protocol for OXCT2 antibody 17122-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Authors - De Huang
View Article