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验证数据展示

  • WB analysis of human kidney using 14943-1-AP

    WB analysis of human kidney using 14943-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 14943-1-AP (OVCA2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 14943-1-AP

    IHC staining of mouse kidney using 14943-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14943-1-AP (OVCA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 14943-1-AP

    IHC staining of mouse kidney using 14943-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14943-1-AP (OVCA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OVCA2 Polyclonal antibody

OVCA2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14943-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

OVCA2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman kidney tissue, human brain tissue, mouse thymus tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14943-1-AP targets OVCA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 OVCA2 fusion protein Ag6744 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 candidate tumor suppressor in ovarian cancer 2
别名 OVCA2
计算分子量 24 kDa
观测分子量 28 kDa
GenBank蛋白编号BC041170
基因名称 OVCA2
Gene ID (NCBI) 124641
RRIDAB_2157286
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WZ82
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for OVCA2 antibody 14943-1-APDownload protocol
IHC protocol for OVCA2 antibody 14943-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols