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OVCA1 Polyclonal antibody

OVCA1 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 20112-1-AP

Print datasheet

Synonyms

DPH like 1, DPH1, DPH1 homolog, DPH1 homolog (S. cerevisiae), DPH2 like 1, DPH2L, DPH2L1, HsDph1, OVCA1

验证数据展示

  • WB analysis of HeLa using 20112-1-AP

    WB analysis of HeLa using 20112-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20112-1-AP (OVCA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20112-1-AP

    IF Staining of HeLa using 20112-1-AP

    Immunofluorescent analysis of HeLa cells using 20112-1-AP (OVCA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20112-1-AP is OVCA1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Tested Reactivity human
Immunogen OVCA1 fusion protein Ag13910 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DPH1 homolog (S. cerevisiae)
Synonyms DPH like 1, DPH1, DPH1 homolog, DPH1 homolog (S. cerevisiae), DPH2 like 1, DPH2L, DPH2L1, HsDph1, OVCA1
Calculated Molecular Weight 489 aa, 52 kDa
Observed Molecular Weight 40-60 kDa
GenBank Accession NumberBC003099
Gene Symbol DPH1
Gene ID (NCBI) 1801
RRIDAB_10641038
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZG8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OVCA1, also named as DPH1, is an evolutionarily conserved gene, indicative of a fundamental biological role in a variety of species. In humans, OVCA1 was initially mapped within the deletion region of 17p13.3 involved in early ovarian cancer (PMID: 8564979). Ovca1-null mice, mainly addressed its tumor suppressive role and potential cooperative role in modifying p53-deficient tumorigenesis (PMID: 14744934). OVCA1 acts as a component of an enzyme complex with DPH2, DPH3 and DPH4 that functionally catalyzes the first step of the diphthamide biosynthesis pathway, to posttranslationally modify a conserved histidine residue (His699 in yeast and His715 in human) of eukaryotic elongation factor 2 (eEF-2).

实验方案

Product Specific Protocols
WB protocol for OVCA1 antibody 20112-1-APDownload protocol
IF protocol for OVCA1 antibody 20112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols