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验证数据展示

  • WB analysis using 87372-1-RR (same clone as 87372-1-PBS)

    WB analysis using 87372-1-RR (same clone as 87372-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87372-1-RR (OTX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87372-1-PBS in a different storage buffer formulation.

  • IF Staining of Y79 using 87372-1-RR (same clone as 87372-1-PBS)

    IF Staining of Y79 using 87372-1-RR (same clone as 87372-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Y79 cells using OTX2 antibody (87372-1-RR, Clone: 252762H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87372-1-PBS in a different storage buffer formulation.

  • Affinity of 87372-1-RR

    Affinity of 87372-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87372-1-RR against Human OTX2 were performed. The affinity constant is 0.132 nM.

OTX2 Recombinant monoclonal antibody, PBS Only

OTX2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87372-1-PBS

产品说明书

CloneNo. 252762H3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

Homeobox protein OTX2, MCOPS5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87372-1-PBS targets OTX2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4323

Product name: Recombinant human OTX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-297 aa of BC032579

Sequence: MMSYLKQPPYAVNGLSLTTSGMDLLHPSVGYPGPWASCPAATPRKQRRERTTFTRAQLDVLEALFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQQQQNGGQNKVRPAKKKTSPAREVSSESGTSGQFTPPSSTSVPTIASSSAPVSIWSPASISPLSDPLSTSSSCMQRSYPMTYTQASGYSQGYAGSTSYFGGMDCGSYLTPMHHQLPGPGATLSPMGTNAVTSHLNQSPASLSTQGYGASSLGFNSTTDCLDYKDQTASWKLNFNADCLDYKDQTSSWKFQVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 orthodenticle homeobox 2
别名 Homeobox protein OTX2, MCOPS5
计算分子量 297 aa, 32 kDa
观测分子量 31-35 kDa
GenBank蛋白编号BC032579
基因名称 OTX2
Gene ID (NCBI) 5015
RRIDAB_3745491
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP32243
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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