验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66276-1-Ig targets OTUD7B in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag10513 Product name: Recombinant human OTUD7B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-355 aa of BC020622 Sequence: MTLDMDAVLSDFVRSTGAEPGLARDLLEGKNWDVNAALSDFEQLRQVHAGNLPPSFSEGSGGSRTPEKGFSDREPTRPPRPILQRQDDIVQEKRLSRGISHASSSIVSLARSHVSSNGGGGGSNEHPLEMPICAFQLPDLTVYNEDFRSFIERDLIEQSMLVALEQAGRLNWWVSVDPTSQRLLPLATTGDGNCLLHAASLGMWGFHDRDLMLRKALYALMEKGVEKEALKRRWRWQQTQQNEESGLVYTEDEWQKEWNELIKLASSEPRMHLGTNGANCGGVESSEEPVYESLEEFHVFVLAHVLRRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYD 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | OTU domain containing 7B |
| 别名 | CEZANNE, OTU domain containing 7B, OTUD7B, ZA20D1, Zinc finger protein Cezanne |
| 计算分子量 | 427aa,48 kDa; 843aa,93 kDa |
| 观测分子量 | 92 kDa |
| GenBank蛋白编号 | BC020622 |
| 基因名称 | OTUD7B |
| Gene ID (NCBI) | 56957 |
| RRID | AB_2881659 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q6GQQ9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OUTD7B is a member of the A20 family of the deubiquitinating cysteine proteases, which has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. It plays a key role in the negative feedback regulation of NF-κB signaling in response to multiple stimuli and has been described as central gatekeeper in inflammation and immunity.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OTUD7B antibody 66276-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature TRAF2 and OTUD7B govern a ubiquitin-dependent switch that regulates mTORC2 signalling.
| ||
Autophagy High-throughput screening of functional deubiquitinating enzymes in autophagy.
| ||
Autophagy OTUD7B deubiquitinates SQSTM1/p62 and promotes IRF3 degradation to regulate antiviral immunity.
| ||
Mol Biol Rep DJ-1 activates the noncanonical NF-κB pathway via interaction with Cezanne to inhibit the apoptosis and promote the proliferation of Ishikawa cells
| ||
J Exp Clin Cancer Res OTUD7B suppresses Smac mimetic-induced lung cancer cell invasion and migration via deubiquitinating TRAF3. | ||
Cell Death Dis OTUD7B stabilizes estrogen receptor α and promotes breast cancer cell proliferation. |