验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human kidney tissue, human heart tissue, mouse kidney tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16605-1-AP targets OTUD7B in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | OTUD7B fusion protein Ag9788 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | OTU domain containing 7B |
| 别名 | CEZANNE, OTU domain containing 7B, OTU7B, OTUD7B, ZA20D1, Zinc finger protein Cezanne |
| 计算分子量 | 427aa,48 kDa; 843aa,93 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC020622 |
| 基因名称 | OTUD7B |
| Gene ID (NCBI) | 56957 |
| RRID | AB_2157149 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6GQQ9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OUTD7B is a member of the A20 family of the deubiquitinating cysteine proteases, which has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. It plays a key role in the negative feedback regulation of NF-κB signaling in response to multiple stimuli and has been described as central gatekeeper in inflammation and immunity. The calculated molecular weight of OTUD7B is 93 kDa, but the modified OTUD7B is about 100 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OTUD7B antibody 16605-1-AP | Download protocol |
| IHC protocol for OTUD7B antibody 16605-1-AP | Download protocol |
| IP protocol for OTUD7B antibody 16605-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature OTUD7B controls non-canonical NF-κB activation through deubiquitination of TRAF3.
| ||
Nat Commun Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation.
| ||
J Exp Med Otud7b facilitates T cell activation and inflammatory responses by regulating Zap70 ubiquitination.
| ||
Circ Res Cezanne regulates inflammatory responses to hypoxia in endothelial cells by targeting TRAF6 for deubiquitination. | ||
Theranostics Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis. | ||
Arterioscler Thromb Vasc Biol Mechanical Activation of Hypoxia-Inducible Factor 1α Drives Endothelial Dysfunction at Atheroprone Sites. |
