OTUB1 Polyclonal antibody
OTUB1 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HepG2 cells, mouse brain tissue, mouse cerebellum tissue, rat brain tissue |
| Positive IF detected in | U2OS cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
10573-1-AP targets OTUB1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | OTUB1 fusion protein Ag0845 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | OTU domain, ubiquitin aldehyde binding 1 |
| Synonyms | Deubiquitinating enzyme OTUB1, hOTU1, HSPC263, OTB1, OTU1, OTUB1, Otubain 1, Ubiquitin thioesterase OTUB1 |
| Calculated Molecular Weight | 31 kDa and 35 kDa |
| Observed Molecular Weight | 31-35 kDa |
| GenBank Accession Number | BC007519 |
| Gene Symbol | OTUB1 |
| Gene ID (NCBI) | 55611 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96FW1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OTUB1 belongs to the ovarian tumor domain protease (OTU) subfamily of deubiquitinases, which could block ubiquitination leading to protein stability. OTUB1 mainly prefers k-48 and k-63 ubiquitin chains type, especially in k-48 ubiquitin chain. OTUB1 performs its deubiquitinating function via two distinct manners: the canonical and non-canonical manner. OTUB1 is involved in many critical immune response processes, containing CD8 T cell activation, PD-L1 related immune evasion, and several infectious diseases. OTUB1 plays an important role in the occurrence, proliferation, invasion, and progression of various cancers, such as bladder cancer, breast cancer, prostate cancer, and colorectal cancer (PMID: 34875341, 35296795). OTUB1 has 2 isoforms with molecular weights of 31 and 35 kDa, respectively. With phospho or ubiquitin modification or reaction, the MW of OTUB1 will be increased.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OTUB1 antibody 10573-1-AP | Download protocol |
| IF protocol for OTUB1 antibody 10573-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Pharmacol Transl Sci Bruceine B Displays Potent Antimyeloma Activity by Inducing the Degradation of the Transcription Factor c-Maf |
