验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, U-251 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33794-1-AP targets OTOG in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg5591 Product name: Recombinant human OTOG protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 1634-1796 aa of NM_001277269.2 Sequence: VRGHGSLPVRTTPPQPSLTASPSSRPVASPGAISRSPTSSGSHKAVLTPAVTKVISRTGVPQPTQAQSASSPSTPLTVAGTAAEQVPVSPLATRSLEIVLSTEKGEAGHSQPMGSPASPQPHPLPSAPPRPAQHTTMATRSPALPPETPAAASLSTATDGLAA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | otogelin |
| 别名 | OTGN, Otogelin |
| 计算分子量 | 315 kDa |
| 观测分子量 | 314 kDa |
| GenBank蛋白编号 | NM_001277269.2 |
| 基因名称 | OTOG |
| Gene ID (NCBI) | 340990 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6ZRI0-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OTOG (Otogelin) is a glycoprotein that is highly expressed in the inner ear and is essential for maintaining hearing and balance functions. It belongs to the mucin-like glycoprotein family and is a large extracellular matrix protein with numerous glycosylation sites. This allows it to form gel-like structures that provide structural support during mechanical transduction in the inner ear. Mutations or loss of function in the OTOG gene can lead to severe hereditary deafness and vestibular dysfunction. There are two isoforms of OTOG, with molecular weights of 160 kDa and 314 kDa respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OTOG antibody 33794-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
